Open AccessSomatic antigens of Pseudomonas aeruginosa
Author(s) -
KNIREL Yuriy A.,
KOCHAROVA Nina A.,
SHASHKOV Alexander S.,
KOCHETKOV Nikolay K.,
KHOLODKOVA Elena V.,
STANISLAVSKY Evgeny S.
Publication year - 1987
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1987.tb13501.x
Subject(s) - pseudomonas aeruginosa , microbiology and biotechnology , polysaccharide , antigen , somatic cell , chemistry , biology , bacteria , biochemistry , immunology , gene , genetics
The lipopolysaccharides from two serologically related strains of Pseudomonas aeruginosa II (Sandvik classification) and V (IM‐1, Verder‐Evans classification) were isolated by the Westphal method and cleaved with 1% acetic acid. The O‐specific polysaccharide from Sandvik II lipopolysaccharide involved L‐rhamnose, N ‐acetyl‐D‐quinovosamine, and N ‐acetyl‐D‐galactosaminuronic acid in the ratio 2:1:1, as well as O ‐acetyl groups. The Verder‐Evans V O‐specific polysaccharide was found to contain just the same sugars and, in addition, D‐glucose, the monosaccharide ratio being 2:1:1:1. On the basis of methylation analysis, Smith degradation, solvolysis with anhydrous hydrogen fluoride and 1 H and 13 C nuclear magnetic resonance data, it was concluded that the polysaccharides have identical main chains, and there were established the following structures of their repeating units:The data obtained allow us to substantiate the combining of the strains studied into one O‐serogroup as two individual O‐serotypes.