Open AccessMonitoring the purification by high‐performance liquid chromatography of cardiotoxins from Naja mossambica mossambica using phase‐sensitive two‐dimensional nuclear magnetic resonance
Author(s) -
OTTING Gottfried,
MARCHOT Pascale,
BOUGIS Pierre E.,
ROCHAT Hervé,
WÜTHRICH Kurt
Publication year - 1987
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1987.tb13459.x
Subject(s) - homogeneous , chromatography , phase (matter) , chemistry , naja , nuclear magnetic resonance , high performance liquid chromatography , materials science , venom , biochemistry , organic chemistry , physics , thermodynamics
High‐resolution phase‐sensitive two‐dimensional proton nuclear magnetic resonance was used to monitor the preparation by high‐performance liquid chromatography of homogeneous proteins from the venom of Naja mossambica mossambica . This resulted in the characterization of a heterogeneous protein preparation V II 2, which had been used in earlier structural studies by NMR, as well as a homogeneous protein CTXIIb and a nearly homogeneous protein fraction CTXIIa, which are now both subject to further investigations of their solution conformations.