Open AccessPolypeptides belonging to each of the three major chlorophyll a + b protein complexes are present in a chlorophyll‐ b ‐less barley mutant
Author(s) -
WHITE Michael J.,
GREEN Beverley R.
Publication year - 1987
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1987.tb11471.x
Subject(s) - chlorophyll , thylakoid , mutant , chlorophyll b , chlorophyll a , photosystem i , light harvesting complexes of green plants , biology , photosystem ii , biochemistry , hordeum vulgare , p700 , chloroplast , photosynthesis , coomassie brilliant blue , staining , botany , poaceae , genetics , gene
Polypeptides of the three major chlorophyll a + b protein complexes were detected in a chlorophyll‐ b ‐less barley mutant (chlorina f2) using immunological techniques. Antibodies to CP Ia, a photosystem I complex containing both the reaction center (CP I) and the chlorophyll a + b antenna (LHCI), detected substantial amounts of LHCI polypeptides in mutant thylakoids. Some polypeptides of the two photosystem‐II‐associated chlorophyll a + b complexes, CP 29 and LHCII, were also detected using antibodies raised against these complexes. The CP 29 apoprotein and the minor 25‐kDa polypeptide of LHCII were present in amounts that could be seen by Coomassie blue staining. In contrast, the two major polypeptides of LHCII were greatly diminished in amount, and one of them may be completely absent. These data suggest that the absence of chlorophyll b may have differing effects on the synthesis, processing or turnover of the various chlorophyll a + b binding polypeptides. They also show that these polypeptides can be inserted into thylakoids in the absence of Chl b , and that significant amounts of some of them are accumulated in the mutant thylakoids.