Open AccessMode of inhibition of the DNA polymerase of Methanococcus vannielii by aphidicolin
Author(s) -
ZABEL HansPeter,
HOLLER Eggehard,
WINTER Josef
Publication year - 1987
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1987.tb11208.x
Subject(s) - aphidicolin , dna polymerase , polymerase , dna clamp , biology , dna , dna polymerase ii , microbiology and biotechnology , biochemistry , dna synthesis , polymerase chain reaction , gene , reverse transcriptase
The mode of action of aphidicolin on DNA synthesis catalysed by the DNA polymerase of Methanococcus vannielii is competitive for dCTP, noncompetitive for dATP, dGTP and dTTP and uncompetitive for activated DNA. The kinetic data are accounted for by a mechanism in which dCTP and aphidicolin compete for the dCTP‐specific binding site on the DNA polymerase. The dissociation constant for the aphidicolin–DNA‐polymerase complex is 0.04–0.07 μM. Similar modes of inhibition of DNA synthesis exist for DNA polymerase α of higher eucaryotes but not for eubacteria or viruses and suggests a close functional relationship between the DNA polymerase of eucaryotes and of the archaebacterium M. vannielii.