Open AccessRecombinant‐DNA‐derived bovine growth hormone from Escherichia coli
Author(s) -
LANGLEY Keith E.,
LAI PorHsiung,
Wypych Jette,
EVERETT Richard R.,
BERG Thor F.,
KRABILL L. F.,
DAVIS Janice M.,
SOUZA Lawrence M.
Publication year - 1987
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1987.tb10803.x
Subject(s) - bovine somatotropin , recombinant dna , chromatography , size exclusion chromatography , sodium dodecyl sulfate , escherichia coli , gel electrophoresis , absorbance , chemistry , circular dichroism , biochemistry , isoelectric focusing , isoelectric point , polyacrylamide gel electrophoresis , enzyme , gene
Bacterially synthesized, recombinant‐DNA‐derived bovine growth hormone (r‐bGH), prepared as described in the preceding paper in this journal, has been characterized in comparison with pituitary bovine growth hormone (pit‐bGH). The characterization criteria include sodium dodecyl sulfate/polyacrylamide gel electrophoresis, automated N‐terminal sequence analysis, amino acid composition, isoelectric focusing, reverse‐phase high‐performance liquid chromatography, ultraviolet absorbance, analysis for free protein thiol, sizing by gel filtration, circular dichroism, radioimmunoassay and biological activity in the hypophysectomized rat weight‐gain assay. In every respect the r‐bGH appears to be virtually identical to pit‐bGH.