Open AccessThe corrinoid from Methanobacterium thermoautotrophicum (Marburg strain)
Author(s) -
KRÄUTLER Bernhard,
MOLL Johanna,
THAUER Rudolf K.
Publication year - 1987
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1987.tb10596.x
Subject(s) - corrinoid , methanobacterium , chemistry , desmethyl , stereochemistry , proton nmr , crystallography , biochemistry , methylation , metabolite , methyltransferase , archaea , gene
The corrinoids from Methanobacterium thermoautotrophicum were extracted as the Co‐cyano derivative, which was isolated in crystalline form. A consistent set of spectroscopic data was acquired (ultraviolet/visible, circular dichroic, infrared, fast‐atom‐bombardment mass, 1 H‐NMR and 13 C‐NMR spectra), which allowed the structural analysis of this complete corrinoid. It was assigned the structure of the Co β ‐cyano‐5′‐hydroxybenzimidazolylcobamide and was identified with Friedrich and Bernhauer's ‘factor III’ by comparison with an authentic sample.