Open AccessQuantification of ATP‐producing and consuming processes of Ehrlich ascites tumour cells
Author(s) -
MÜLLER Marianne,
SIEMS Werner,
BUTTGEREIT Frank,
DUMDEY Renate,
RAPOPORT Samuel Mitja
Publication year - 1986
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1986.tb10496.x
Subject(s) - proteolysis , respiration , biochemistry , protein biosynthesis , atpase , cellular respiration , atp synthase , ehrlich ascites , chemiosmosis , chemistry , rna , amino acid , biology , enzyme , mitochondrion , tumor cells , botany , cancer research , gene
ATP production of Ehrlich ascites tumour cells was estimated on the basis of their coupled respiration and lactate formation. ATP‐consuming processes were assessed from the effects of selective inhibitors of RNA synthesis, protein synthesis and proteolysis, Na + /K + ‐ATPase and Ca 2+ ‐ATPase on respiration. The extent of protein synthesis and proteolysis were also determined directly. From these values and those of the inhibition of respiration by selective inhibitors, a P/O ratio of 2.2 was calculated. About 75% of the total ATP consumption could be assigned to specific processes. The major ATP‐consuming processes of tumour cells in an amino‐acid‐enriched medium, in which they are in an approximate steady state, are protein synthesis with about 30% of total ATP consumption, and Na + /K + ‐ATPase with about 20%, while RNA synthesis, ATP‐dependent proteolysis and Ca 2+ ‐ATPase contribut about 10% each. In an amino‐acid‐free glucose medium, protein synthesis is reduced to a third, with a corresponding decrease of respiration, whereas the rate of the other ATP‐consuming processes is unchanged.