Open AccessNucleotide sequence of a cDNA clone encoding Scylliorhinus caniculus protamine Z2
Author(s) -
BERLOTPICARD Frederique,
VODJDANI Guilane,
DOLY Janine
Publication year - 1986
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1986.tb09972.x
Subject(s) - protamine , complementary dna , nucleic acid sequence , microbiology and biotechnology , biology , coding region , peptide sequence , consensus sequence , sequence (biology) , messenger rna , genetics , stop codon , amino acid , biochemistry , dna , gene , heparin
A cDNA library was constructed from a protamine‐enriched fraction of dogfish ( Scylliorhinus caniculus ) mRNA. The nucleotide sequence of a 440‐bp insert was determined, and its produced protein sequence confirmed its identification as a cysteine‐rich protamine Z2 [Martinage, A., Gusse, M., Belaiche, D., Sautiere, P. and Chevaillier, P. (1985) Biochim. Biophys. Acta 831 , 172–178]. The frequency of utilization of the different triplets coding for arginine, which represents 30–70% of the total amino acid residues for trout, mouse and dogfish protamines, is discussed. An alternative repetitive sequence of CGC‐AGG was found in the N terminus of the protein. Analysis of the 3′ flanking region after the mRNA‐terminating TAA codon identified an inverted repeat sequence and an ACCA sequence, which may be possible vestiges of a histone‐like termination signal.