Open AccessThe structure of the O ‐glycosidic oligosaccharide chains of the major Zajdela hepatoma ascites‐cell‐membrane glycoprotein
Author(s) -
NATO Farida,
GOULUT Chantal,
BOURRILLON Roland,
HALBEEK Herman,
VLIEGENTHART Johannes F. G.
Publication year - 1986
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1986.tb09868.x
Subject(s) - glycoprotein , glycosidic bond , ascites , oligosaccharide , chemistry , biochemistry , microbiology and biotechnology , biology , medicine , enzyme
Glycoprotein MII 2 , the major cell surface glycoprotein (molecular mass 110 kDa) of Zajdela hepatoma ascites cells, contains about 25 O ‐glycosidic oligosaccharide chains per molecule. They were released as oligosaccharidealditols by alkaline borohydride treatment of MII 2 , and purified by gel filtration on Bio‐Gel P‐6 followed by high‐voltage paper electrophoresis. Four oligosaccharide‐alditol fractions (A–D) were obtained in relative yields of 8:6:3:3. The structure of the components of fractions A–C was determined by 500‐MHz 1 H‐NMR spectroscopy in combination with sugar composition analysis, to be as follows.On the basis of sugar composition and characteristics on Bio‐Gel P‐6 filtration, paper electrophoresis and thin‐layer chromatography, the structure of the carbohydrate component of fraction D is proposed to be as follows.