Open AccessPrimary structure of the neutral carbohydrate chains of hemocyanin from Panulirus interruptus
Author(s) -
KUIK J. Albert,
HALBEEK Herman,
KAMERLING Johannis P.,
VLIEGENTHART Johannes F. G.
Publication year - 1986
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1986.tb09867.x
Subject(s) - glycosidic bond , mannose , hemocyanin , carbohydrate , chemistry , fractionation , pronase , chromatography , biochemistry , biology , trypsin , antigen , genetics , enzyme
The N ‐glycosidic carbohydrate chains of Panulirus interruptus hemocyanin, consisting of only mannose and N ‐acetylglucosamine residues, were liberated by hydrazinolysis of a pronase digest of the purified glycoprotein. The carbohydrate chains were fractionated by high‐voltage paper electrophoresis, yielding mainly neutral oligosaccharide‐alditols. These were further separated by high‐performance liquid chromatography and characterized by 500‐MHz 1 H‐NMR spectroscopy as