Open AccessIsolation and characterization of a 2.2‐kb operon preceding the α‐amylase gene of Bacillus amyloliquefaciens
Author(s) -
KALLIO Pauli,
ULMANEN Ismo,
PALVA Ilkka
Publication year - 1986
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1986.tb09782.x
Subject(s) - bacillus amyloliquefaciens , operon , gene , microbiology and biotechnology , plasmid , biology , bacillus subtilis , base pair , dna , amylase , genetics , enzyme , escherichia coli , biochemistry , bacteria
A DNA region of 2.8 × 10 3 base pairs (2.8 kb) upstream of the Bacillus amyloliquefaciens α‐amylase gene has been isolated. This DNA gave rise to a 2.2‐kb transcript. The 3′ end of the transcript was mapped with S1 nuclease and shown to terminate 49 base pairs upstream of the‐35 region of the α‐amylase promoter. In B. subtilis minicells this 2.2‐kb transcript coded for three different polypeptides, thus indicating a polycistronic operon‐type structure. The location and the order of the polypeptides were established using DNA deletions. The joining of the 2.2‐kb operon to the downstream α‐amylase gene in the plasmid pUB110 did not have any significant effect on the level of expression of the α‐amylase.