Open AccessRegulation of type I collagen mRNA levels in fibroblasts
Author(s) -
VOSS Tilman,
BORNSTEIN Paul
Publication year - 1986
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1986.tb09686.x
Subject(s) - polysome , procollagen peptidase , messenger rna , rna , microbiology and biotechnology , biology , translation (biology) , protein biosynthesis , chemistry , ribosome , biochemistry , gene
Type I procollagen mRNA levels, as well as total RNA and poly(A)‐rich mRNA, remain constant when rapidly growing human fetal lung fibroblasts (HFL‐1 cells) are compared with quiescent cells. Polysome profiles of cells in both growth states revealed that the distribution of type I collagen mRNA in the mRNP fraction and in polysomes also remained constant even though total RNA and poly(A)‐rich mRNA were shifted from polysomes to the mRNP pool in resting cells. Similar results were obtained when RNA fractions in polysomes associated with the cytoskeletal framework were examined. It is known that procollagen production is unaffected by the growth state of cells [Breul, S. D., Bradley, K. H., Hance, A. J., Schafer, M. P., Berg, R. A. and Crystal, R. G. (1980) J. Biol. Chem. 255 , 5250–5260] although total protein synthesis is markedly decreased in resting cells. It would therefore appear that the translational control responsible for reduced synthesis of non‐collagenous proteins in resting cells does not extend to procollagen and that transcriptional control can account for levels of type I procollagen produced by cultured human fibroblasts.