Open AccessPrimary structures of mutationally altered ribosomal protein L7/L12 and their effects on cellular growth and translational accuracy
Author(s) -
KIRSEBOM Leif A.,
AMONS Reinout,
ISAKSSON Leif A.
Publication year - 1986
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1986.tb09630.x
Subject(s) - ribosome , mutant , ribosomal protein , biology , point mutation , nonsense mutation , translation (biology) , missense mutation , genetics , start codon , ribosomal rna , protein biosynthesis , microbiology and biotechnology , mutation , gene , messenger rna , rna
The amino acid sequences of mutationally altered ribosomal protein L7/L12 from four different rplL mutants of Escherichia coli were determined and correlated with some features of the mutant ribosomes. Two of the rplL mutations are deletions around position 40, which give rise to a shortened hinge region between the two domains of L7/L12. The other two mutants harbor point mutations at position 74 (Gly → Asp) or at position 82 (Glu → Lys), which are in or close to an evolutionarily conserved sequence in the C‐terminal domain. The two latter mutations are associated with decreased rates of growth and translational elongation. All four mutants show increased nonsense codon read‐through in vivo . Ribosomes from one of the deletion mutants show clearly increased missense error rates in vitro .