Open AccessInhibition of dolichyl phosphate biosynthesis by compactin in cultured rat hepatocytes
Author(s) -
ÅSTRAND IngMari,
FRIES Erik,
CHOJNACKI Tadeusz,
DALLNER Gustav
Publication year - 1986
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1986.tb09511.x
Subject(s) - biosynthesis , reductase , biochemistry , phosphorylation , chemistry , incubation , coenzyme a , enzyme , phosphate , kinase , cell culture , microbiology and biotechnology , biology , genetics
Isolated rat hepatocytes were cultured in monolayer for about 24 h. During this period the cells exhibited constant protein and lipid synthesis. When the culture medium contained compactin, a competitive inhibitor of the 3‐hydroxyl‐3‐methylglutary‐coenzyme‐A reductase, dolichyl‐ P synthesis was inhibited by 91% at the end of the incubation, as estimated by the incorporation of [ 3 H]acetate and by 77% as estimated by the incorporation of 32 P i . These results indicate that in primary cultures of rat hepatocytes dolichyl monophosphate is mainly synthesized through a de novo process, while phosphorylation through the CTP‐mediated kinase is of limited functional importance.