Open AccessNew phase supports for liquid‐liquid partition chromatography of biopolymers in aqueous poly(ethyleneglycol)‐dextran systems
Author(s) -
MÜLLER Werner
Publication year - 1986
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1986.tb09479.x
Subject(s) - chromatography , aqueous solution , dextran , cellulose triacetate , chemistry , phase (matter) , cellulose , partition coefficient , polymer chemistry , partition (number theory) , chemical engineering , organic chemistry , mathematics , combinatorics , engineering
New phase supports for liquid‐liquid partition chromatography, using aqueous poly(ethyleneglycol)‐dextran systems have been developed by grafting linear polyacrylamide chains on to premanufactured chromatographic supports carrying primary or secondary aliphatic hydroxyl functions on their surface. Columns prepared from such supports have a higher binding capacity for the dextran‐rich stationary phase and much higher performances than columns prepared from cellulose, the previously used phase support for this system. Test separations of DNA restriction fragments, ranging from 11 base pairs to 3829 base pairs, document a high resolution for DNA fragments larger than 200 base pairs.