Open AccessPreferential conformation of substance P in solution
Author(s) -
CHASSAING Gérard,
CONVERT Odile,
LAVIELLE Solange
Publication year - 1986
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1986.tb09361.x
Subject(s) - chemistry , circular dichroism , monomer , aqueous solution , nuclear magnetic resonance spectroscopy , pyridine , methanol , stereochemistry , crystallography , substance p , medicinal chemistry , organic chemistry , polymer , biochemistry , receptor , neuropeptide
The three‐dimensional structure of substance P has been studied by 1 H‐NMR, (500 MHz), and by circular dichroism (CD) in different solvents. The analysis of the different NMR parameters suggest that substance P adopts a rather extended structure in dimethylsulfoxide and pyridine. In water, besides the aggregation phenomenon, the monomeric substance P presents a complex conformational equilibrium. The addition of sodium dodecylsulfate to the aqueous solution induces, as shown by CD spectroscopy, a preferential α‐helical conformation. And in methanol three structural conclusions may be drawn: the flexibility of the N‐terminal Arg‐Pro‐Lys, the α‐helical structure of Pro 4 ‐Gln 5 ‐Gln 6 ‐Phe 7 ‐Phe 8 and the interaction of the C‐terminal carboxamide with the primary amides from both glutamines.