Open AccessAnalysis of modification‐dependent structural alterations in the anticodon loop of Escherichia coli tRNA Arg and their effects on the translation of MS2 RNA
Author(s) -
BAUMANN Ulrich,
FISCHER Wolfgang,
SPRINZL Mathias
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1985.tb09243.x
Subject(s) - transfer rna , escherichia coli , translation (biology) , rna , nucleoside , ribose , nuclease , biology , biochemistry , chemistry , dna , enzyme , messenger rna , gene
The conformation of the anticodon loop of Escherichia coli tRNA Arg was investigated. It is shown that the structure of the anticodon loop is influenced by the base composition of the anticodon stem, and the natural modification of the nucleoside residue 32 in the anticodon loop. The structural effects detected by analysis of the accessibility of the anticodon loop to nuclease S1 could be correlated with the ability of different Arg‐tRNA Arg species to suppress frame‐shifting during translation of MS2 RNA.