Open AccessSequence analysis of a cloned cDNA coding for bovine seminal ribonuclease
Author(s) -
PALMIERIE Marta,
CARSANA Antonella,
FURIA Adriana,
LIBONATI Massimo
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1985.tb09194.x
Subject(s) - complementary dna , ribonuclease , coding region , bovine pancreatic ribonuclease , pancreatic ribonuclease , microbiology and biotechnology , messenger rna , biology , nucleic acid sequence , peptide sequence , nucleotide , sequence analysis , gene , rna , biochemistry
The sequence of a cloned cDNA coding for bovine seminal ribonuclease, an enzyme secreted in the bull seminal vesicles, was determined. The cDNA starts at the amino acid residue 47 and terminates 12 nucleotides beyond the consensus sequence AAUAAA in the 3′ non‐coding region of the mRNA. Northern blotting analysis shows that the mRNA for bovine seminal ribonuclease consists of about 950 nucleotides, a value that is similar to that of other mRNAs coding for ribonucleases of the pancreatic type.