Open AccessStructural studies on the oligosaccharide moety of the lipopeptidophosphoglycan from Trypanosoma cruzi
Author(s) -
LEDERKREMER Rosa M.,
CASAL Olga L.,
COUTO Alicia,
COLLI Walter
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1985.tb09136.x
Subject(s) - trypanosoma cruzi , galactose , hydrolysate , oligosaccharide , chemistry , biochemistry , moiety , mannose , glucosamine , stereochemistry , monosaccharide , ceramide , n acetylglucosamine , acid hydrolysis , fucose , nuclear magnetic resonance spectroscopy , hydrolysis , enzyme , parasite hosting , computer science , apoptosis , world wide web
The structure of the carbohydrate moiety of the lipopeptidophosphoglycan from Trypanosoma cruzi was studied by 13 C NMR spectroscopy and by methylation analysis of the original and of an acid‐degraded sample. An oligosaccharide, consisting of 2‐ O ‐substituted and 6‐ O ‐substituted mannoses, which is linked to the ceramide, was separated by partial acid hydrolysis from an external chain that contained 3‐ O ‐substituted mannopyranosyl residues. β‐ D ‐Galactofuranosyl terminal units are attached to position 3 of (1 → 2)‐linked mannopyranose. Besides the previously reported monosaccharide components (mannose, galactose, glucose and glucosamine), ribose was identified in a partial acid hydrolysate of the lipopeptidophosphoglycan. The last three sugars are minor components and their oganization into the overall structure of the lipopeptidophosphoglycan has not been determined.