Open AccessInitiation factor eIF2 associated with non‐polysomal poly(A)‐containing messenger ribonucleoproteins of cryptobiotic gastrulae of Artemia salina
Author(s) -
HERDT Erik,
PIOT Etienne,
WAHBA Albert,
SLEGERS Herman
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1985.tb09123.x
Subject(s) - ribonucleoprotein , biology , messenger rnp , polysome , eif2 , artemia salina , biochemistry , initiation factor , rna , microbiology and biotechnology , ribosome , chemistry , organic chemistry , toxicity , gene
Non‐polysomal poly(A)‐containing mRNP of A.salina cryptobiotic embryos is separated in mRNP active in protein synthesis and in repressed mRNP by sucrose gradient centrifugation. In the translationally active fraction the presence of eukaryotic initiation factor 2 (eIF2) is demonstrated by electroblotting of sodium dodecylsulphate/polyacrylamide gels on nitrocellulose and anti‐eIF2 antibody detection. mRNP proteins with M r of 40000 and 42000 are identified as the α and β subunits of eIF2. The repressed mRNP is devoid of eIF2 and is associated with an inhibitor ribonucleoprotein composed of a small 85 ± 2‐nucleotide‐long RNA and a protein with M r of 64000. The latter ribonucleoprotein is a potent inhibitor of the translationally active mRNP.