Open AccessLipopolysaccharides from Yersinia pestis
Author(s) -
DALLA VENEZIA Nicole,
MINKA Samuel,
BRUNETEAU Maud,
MAYER Hubert,
MICHEL Georges
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1985.tb09115.x
Subject(s) - disaccharide , lipid a , chemistry , glucosamine , lipopolysaccharide , residue (chemistry) , yersinia pestis , phosphate , stereochemistry , heptose , biochemistry , virulence , biology , gene , mutant , endocrinology
The chemical structure of the lipid A of lipopolysaccharide I and II from Yersinia pestis , strain EV 40, was studied. It consists of a (1 → 6), β‐linked d ‐glucosamine disaccharide which carries two phosphate groups; one phosphate is linked glycosidically with a glucosamine unit, the other one is linked to the non‐reducing glucosamine. Various degradation methods combined with 31 P nuclear magnetic resonance spectroscopy showed that the esterbound phosphate group is linked to a 4‐aminoarabinosyl residue and the glycosidically linked phosphate group is linked to a d ‐arabinofuranosyl residue in lipopolysaccharide II and to the phosphorylethanolamine in lipopolysaccharide I. The hydroxyl groups of the disaccharide are acylated by dodecanoic, hexadecenoic, 3‐hydroxytetradecanoic and 3‐dodecanoyloxytetradecanoic acids. The amino groups of the disaccharide carry 3‐hydroxytetradecanoic and 3‐dodecanoyloxytetradecanoic acids. In addition smaller amounts of 3‐tetradecanoyloxyltetradecanoic and 3‐hexadecanoyloxytetradecanoic acids are present in ester linkage.