Open AccessSomatic antigens of Pseudomonas aeruginosa
Author(s) -
KNIREL Yurij A.,
SKVORTSOV Igor M.,
SHASHKOV Alexander S.,
DMITRIEV Boris A.,
KOCHETKOV Nikolay K.,
STANISLAVSKY Evgeny S.,
MASHILOVA Galina M.
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1985.tb09056.x
Subject(s) - polysaccharide , monosaccharide , chemistry , rhamnose , galactose , pseudomonas aeruginosa , sephadex , residue (chemistry) , strain (injury) , stereochemistry , biochemistry , bacteria , biology , enzyme , anatomy , genetics
Lipopolysaccharides were isolated from the phenol layer on aqueous phenol extraction of cells of Pseudomonas aeruginosa 011 (Lányi classification), strains 170021 and 170040. On mild acid degradation of the lipopolysaccharides, with the subsequent gel‐filtration on Sephadex G‐50, neutral O‐specific polysaccharides made up of 6‐deoxysugars alone were obtained. Two 2‐acetamido‐2,6‐dideoxy‐L‐galactose (LFucNAc), 2‐acetamido‐2,6‐dideoxy‐D‐glucose (DQuiNAc) and L‐rhamnose (LRha) residues were found to be the components of the strain 170021 polysaccharide repeating units; those of strain 170040 contained the same monosaccharides, but, instead of 2‐acetamido‐2,6‐dideoxy‐D‐glucose residue, that of 2‐acetamido‐2,6‐dideoxy‐D‐galactose (DFucNAc) was present. On the basis of the 13 C nuclear magnetic resonance data, methylation analysis and three successive Smith degradations the following structures were determined for the polysaccharide repeating units: strain 170021. →2)LRha(α1→3)LFucNAc(α1→3)LFucNAc(α1→3)DQuiNAc(β1→; strain 170040, →2)LRha(α1→3)LFucNAc‐ (α1→3)LFucNAc(α1→3)DFucNAc(β1→; differing from one another by configuration of C‐4 of 2‐acetamido‐2,6‐dideoxy‐D‐hexopyranose only.