Open AccessIdentification of multiple variants of fast muscle troponin T in the chicken using monoclonal antibodies
Author(s) -
BIRD Ian M.,
DHOOT Gurtej K.,
WILKINSON J. Michael
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1985.tb09052.x
Subject(s) - microbiology and biotechnology , monoclonal antibody , troponin , troponin t , biology , antibody , troponin c , immunostaining , immunohistochemistry , immunoperoxidase , troponin complex , immunology , medicine , myocardial infarction
Two monoclonal antibodies, T1/7 and T1/61, have been prepared which are specific for chicken fast muscle troponin T. Both are of the IgGγ1 subclass. Both antibodies cross‐react strongly with human fast and chicken cardiac troponin T, but while T1/7 reacts weakly with rabbit fast troponin T, T1/61 does not. The antibodies can be used for fibre typing of both chicken and human muscle. The antibodies have been used to identify fast troponin T on two‐dimensional maps of proteins from a variety of chicken muscles by electrophoretic transfer to nitrocellulose followed by immunoperoxidase staining. Using this technique five variant forms of fast troponin T have been identified. Two variants, fBT 1 and fBT 2 , are expressed in breast muscle, while the other three, fLT 1 , fLT 2 and fLT 3 are expressed in leg muscle. Of the leg muscle variants, fLT 1 and fLT 2 correspond to the two forms described previously [Wilkinson, J. M., Moir, A. J. G. and Waterfield, M. D. (1984) Eur. J. Biochem. 143 , 47–56]. The third variant, fLT 3 , has not been described before and is expressed in muscles which have a high content of slow fibres. In addition to these clearly defined variant forms immunostaining reveals multiple minor variants of troponin T present in leg muscle which may reflect complex RNA processing of the troponin T gene transcript.