Open AccessCloning and nucleotide sequence of the murine interleukin‐3 gene
Author(s) -
CAMPBELL Hugh D.,
YMER Sanie,
FUNG Mingchiu,
YOUNG Ian G.
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1985.tb09020.x
Subject(s) - microbiology and biotechnology , biology , gene , ecori , coding region , nucleic acid sequence , complementary dna , genomic library , intron , exon , genetics , genomic dna , peptide sequence , restriction enzyme
Southern hybridization analysis using a probe derived from a murine interleukin‐3 (IL‐3) cDNA clone revealed the presence of a single IL‐3 gene in the haploid murine genome. An 8600‐base‐pair (8.6‐kb) murine genomic Eco RI fragment containing the IL‐3 gene was isolated by screening a library of size‐fractionated genomic Eco RI fragments cloned in λgt WES. λB. The nucleotide sequence of a 3.5‐kb region of the cloned DNA encompassing the IL‐3 gene was determined. The gene contains four introns of 96, 993, 135 and 122 base pairs (bp), located within the coding region. The large intron contains 12 copies of a 14–15‐bp tandem repeating sequence which resemles a human cellular homologue of a BKV enhancer sequence. The nucleotide sequence of the exons agrees exactly with that of an IL‐3 cDNA cloned from WEHI‐3, a tumorigenic cell line which over‐produces IL‐3, establishing that the unprocessed primary structure of IL‐3 is identical in WEHI‐3 and in BALB/c mice. Southern hybridization has revealed genomic alteration in the vicinity of the IL‐3 gene in WEHI‐3 cells.