Open AccessPhoto‐induced protein cross‐linking to 5S RNA and 28–5.8S RNA within rat‐liver 60S ribosomal subunits
Author(s) -
PALEOLOGUE Anne,
REBOUD AnneMarie,
REBOUD JeanPaul
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1985.tb08956.x
Subject(s) - rna , ribosomal rna , 5s ribosomal rna , microbiology and biotechnology , eukaryotic large ribosomal subunit , ribosomal protein , protein subunit , biochemistry , ribosome , chemistry , biology , gene
Rat liver 60S ribosomal subunits were irradiated with 254‐nm ultraviolet light (1.26 × 10 4 quanta/subunit), under conditions which preserved their functional activity. Cross‐linked RNA‐protein complexes were recovered after unreacted proteins had been removed by repeated acetic acid extractions. Proteins linked to the whole rRNA, to 5S RNA and to 28–5.8S RNAs were identified by two‐dimensional gel electrophoresis after RNA hydrolysis by ribonucleases T 1 and A. Our results showed that numerous proteins interact with rRNAs (at least ten with 28–5.8S RNA, eight with 5S RNA and among these three are common to both) and have been discussed in the light of all the available data.