Open Access31 P nuclear magnetic resonance studies of crayfish (Orconectes virilis)
Author(s) -
BUTLER Keith W.,
DESLAURIERS Roxanne,
GEOFFRION Yves,
STOREY Janet M.,
STOREY Kenneth B.,
SMITH Ian C. P.,
SOMORJAI Rajmund L.
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1985.tb08896.x
Subject(s) - crayfish , kinetics , in vivo , nuclear magnetic resonance , enzyme kinetics , physics , biophysics , chemistry , enzyme , biology , ecology , active site , microbiology and biotechnology , quantum mechanics
31 P nuclear‐magnetic resonance (NMR) has been used to observe in vivo the steady‐state levels of phosphoruscontaining metabolites in the crayfish Orconectes virilis and the intracellular pH of the abdominal muscle was determined. Measurement of spin‐lattice relaxation times and spin transfer experiments have enabled calculation of unidirectional rate constants and activation energies for the arginine kinase reaction in vivo.