Open AccessThe design of fluorescent probes which bind to the active centre of guanidinobenzoatase
Author(s) -
STEVEN Frank S.,
GRIFFIN Margaret M.,
ALAHMAD Rajaa K.
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1985.tb08889.x
Subject(s) - fluorescence , enzyme , fluorescence microscope , chemistry , active site , biochemistry , non competitive inhibition , combinatorial chemistry , biophysics , stereochemistry , biology , physics , quantum mechanics
Cells possessing a known enzymic activity may be located by fluorescent probes designed to act as competitive inhibitors of this enzyme. We have prepared a series of dansyl N ‐substituted guanidino derivatives which bind to the active centre of guanidinobenzoatase. 9‐Aminoacridine also acts as a competitive inhibitor and behaves similarly to these guanidino derivatives. These fluorescent probes have been used to locate tumour cells possessing this enzyme in thin sections of fixed tissue by employing fluorescent microscopy.