Open AccessBiphasic increase in the in vivo phosphorylation of nuclear 110‐kDa protein during early lymphocyte transformation
Author(s) -
KOSTKA Günter,
SCHWEIGER Anton
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1985.tb08858.x
Subject(s) - in vivo , phosphoprotein , phosphorylation , concanavalin a , microbiology and biotechnology , dna synthesis , biology , chemistry , in vitro , biochemistry , genetics
A major 110‐kDa phosphoprotein in rat liver and hepatoma (P 110) was identified in nuclear 0.2 M HCl extracts from rat lymph node cells. Stimulation with concanavalin A altered both the amount of P110 and, more strikingly, its in vivo phosphorylation in a typical biphasic manner with an initial maximum after 10–14 h. RNA synthesis showed a similar biphasic increase. Inhibition of hnRNA synthesis (5,6 dichlorobenzimidazoleriboside), but not of DNA synthesis (hydroxyurea), depressed both the amount of P110 and its phosphorylation markedly.