Open AccessIn vitro transcription initiation from three different Escherichia coli promoters
Author(s) -
EHRLICH Ricardo,
LAROUSSE Annick,
JACQUET MarieAnge,
MARIN Monica,
REISS Claude
Publication year - 1985
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1985.tb08838.x
Subject(s) - dna supercoil , promoter , rna polymerase , transcription (linguistics) , microbiology and biotechnology , polymerase , lac repressor , biology , dna , plasmid , chemistry , escherichia coli , genetics , lac operon , gene , gene expression , dna replication , linguistics , philosophy
Transcription initiation from β ‐lactamase, tetracycline resistance and RNA1 promoters, present in plasmid pAT 153, were studied employing the abortive initiation technique. Assays appear to be promoter‐specific with supercoiled and linear templates. Supercoiling enhances the isomerization rate constant of the open RNA‐polymerase–promoter complex formation. Results agree with the in vivo behaviour of the corresponding promoters, and allow us to propose a hypothesis about the effect of supercoiling on transcription initiation.