Open AccessThe complete amino acid sequence of the major α subunit of the lectin from the seeds of Dioclea grandiflora (Mart)
Author(s) -
RICHARDSON Michael,
CAMPOS Francisco D. A. P.,
MOREIRA Renato A.,
AINOUZ Iracaema L.,
BEGBIE Robert,
WATT William B.,
PUSZTAI Arpad
Publication year - 1984
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1984.tb08436.x
Subject(s) - lectin , traditional medicine , protein subunit , peptide sequence , chemistry , sequence (biology) , botany , biochemistry , biology , medicine , gene
The complete amino acid sequence of the major a subunit of the lectin from seeds of Dioclea grandiflora was determined. The sequence was deduced from analysis of peptides derived from the native α subunit by digestion with trypsin, chymotrypsin, the Staphylococcus aureus V8 protease, and pepsin; and from larger peptides produced by digestion of the citraconylated protein with trypsin. The α subunit consists of a single polypeptide chain of 237 amino acids which differs from the sequence of concanavalin in 53 positions. Significant levels of heterogeneity were observed in five positions in the sequence.