Open AccessNucleotide sequence of the gene for the immunity protein to colicin A
Author(s) -
LLOUBES Roland P.,
CHARTIER Marline J.,
JOURNET Agnes M.,
VARENNE Stanislas G.,
LAZDUNSKI Claude J.
Publication year - 1984
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1984.tb08432.x
Subject(s) - colicin , operon , gene , genetics , biology , codon usage bias , nucleic acid sequence , start codon , nucleotide , peptide sequence , bacteriocin , amino acid , sequence (biology) , molecular evolution , stop codon , genome , escherichia coli , bacteria
The nucleotide sequence of the structural gene for the immunity protein to colicin A ( cai ) has been established. This sequence consists of 534 base pairs. According to the predicted amino acid sequence, the polypeptide chain of this immunity protein comprises 178 amino acids and has a relative molecular mass of 20462. As expected from its localization in the inner membrane, large hydrophobic fragments are found along the polypeptide chain that also contains clusters of mostly positively charged residues. The cai like the ceiA genes encode proteins that are weakly expressed as compared to the corresponding colicins (A and E1). Codon usage reflects this difference. In contrast, the four genes for immunity to cloacin DF13 and to colicin E3 and for these bacteriocins, all of which are highly expressed and are organized in operon, display similar codon usage. These results are discussed with regards to the possible relationship between expressivity and codon usage.