Open AccessFurther characterization, by a combined high‐performance liquid chromatography/ 1 H‐NMR approach, of the heterogeneity displayed by the neutral carbohydrate chains of human bronchial mucins
Author(s) -
LAMBLIN Geneviève,
BOERSMA Arnold,
LHERMITTE Michel,
ROUSSEL Philippe,
MUTSAERS Johanna,
HALBEEK Herman,
VLIEGENTHART Johannes
Publication year - 1984
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1984.tb08363.x
Subject(s) - mucin , carbohydrate , chemistry , chromatography , characterization (materials science) , high performance liquid chromatography , biochemistry , materials science , nanotechnology
From bronchial mucins of cystic fibrosis patients with blood group O, the carbohydrate chains were released in the form of oligosaccharide‐alditols by alkaline borohydride treatment. Application of high‐performance liquid chromatography directly on the pool of neutral oligosaccharides afforded 23 fractions. Twenty oligosaccharide structures were characterized by employing 500‐MHz 1 H‐NMR spectroscopy in conjunction with sugar analysis. Thirteen among these had been revealed before to occur in human bronchial mucins [Van Halbeek, H., Dorland. L., Vliegenthart, J. F. G., Hull, W. E., Lamblin, G., Lhermitte, M., Boersma, A., and Roussel, P. (1982) Eur. J. Biochem. 127 , 7–20], when paper‐chromatographic fractionation of this pool of neutral oligosaccharides was employed. High‐performance liquid chromatography enabled to obtain another seven pentasaccharide and hexasaccharide alditols; the largest‐size representatives are:Thereby, this approach afforded deeper insight into the structural heterogeneity displayed by the carbohydrate chains of bronchial mucins.