Open AccessThe primary structure of teichuronic acid in Bacillus subtilis AHU 1031
Author(s) -
YONEYAMA Toshie,
ARAKI Yoshio,
ITO Eiji
Publication year - 1984
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1984.tb08160.x
Subject(s) - bacillus subtilis , chemistry , chromium trioxide , polysaccharide , glycosidic bond , carbohydrate conformation , nuclear magnetic resonance spectroscopy , periodate , biochemistry , stereochemistry , organic chemistry , bacteria , enzyme , biology , chromium , genetics
Structural studies were carried out on the acidic polysaccharide fraction obtained from lysozyme digest of the cell walls of Bacillus subtilis AHU 1031. The polysaccharide fraction contained N ‐acetylmannosaminuronic acid (ManNAcA), N ‐acetylglucosamine (GlcNAc), glucose, glycerol and phosphorus in a molar ratio of 2:2:4:1:1, together with glycopeptide components. The results of analyses involving Smith degradation, chromium trioxide oxidation, methylation and proton magnetic resonance spectroscopy led to the conclusion that the backbone chain of the polysaccharide has the repeating unit → 6)Glc(α1 → 3/4)ManNAcA(β1 → 4)GlcNAc(β1 →. About 50% of the N ‐acetylglucosamine residues in the backbone chain seem to be substituted at C‐3 by the glycosidic branches, glycerol phospho‐6‐glucose, while the other half seem to be substituted by glucose.