Open AccessAccounting for the ATP‐consuming processes in rabbit reticulocytes
Author(s) -
SIEMS Werner,
DUBIEL Wolfgang,
DUMDEY Renate,
MÜLLER Marianne,
RAPOPORT Samuel Mitja
Publication year - 1984
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1984.tb07982.x
Subject(s) - reticulocyte , proteolysis , cycloheximide , biochemistry , atp synthase , chemiosmosis , lysine , protein biosynthesis , chemistry , biology , microbiology and biotechnology , rna , gene , enzyme , amino acid
The report deals with a detailed balance of ATP production and consumption of the rabbit reticulocyte. 1. The sum‐total of ATP produced amounts to 135mmol · l −1 · h −1 . 2. About 70% of the ATP consumption has been accounted for by specific processes. The main contributing processes are the globin synthesis with about 28%, the Na + , K + ‐ATPase with 23% and the proteolysis with more than 15%. 30% of ATP consumption has not been accounted for. 3. Cycloheximide (20 μM) leads to a dissociation between synthesis and degradation of proteins, which argues against any obligatory connection between these processes. 4. More than 90% of the lysine liberated from mitochondria by proteolysis were reutilized for the globin synthesis demonstrating the high nitrogen economy of reticulocytes. 5. Each of the ATP‐consuming processes studied appears to control ATP production in an independent manner without competition with each other.