Open AccessExodeoxyribonuclease from rat brain specific for single‐stranded DNA
Author(s) -
IVANOV Vladimir A.,
GAZIEV Azhub I.,
TRETYAK Tatyana M.
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07856.x
Subject(s) - dna , sephadex , heterologous , biochemistry , cofactor , chemistry , enzyme , electrophoresis , microbiology and biotechnology , nucleoside , agarose , hydrolysis , uridine , chromatography , biology , rna , gene
An exodeoxynuclease with single‐stranded DNA specificity has been isolated from rat brain and purified to electrophoretic homogeneity. Approximately 1100‐fold purification with a yield of 16% has been achieved by chromatography on DNA‐agarose, hydroxyapatite and Sephadex G‐200. The enzyme has a pH optimum at 8.4, requires Mg 2+ or Mn 2+ as a cofactor and has a molecular weight of about 60 000. It hydrolyzes homologous, heterologous, synthetic and depurinated substrates at the same rate liberating nucleoside 5′‐monophosphates but does not attack ultraviolet‐irradiated polydeoxyribonucleotides. This DNase is localized predominantly in neuronal cell nuclei and appears to be lacking in rat liver tissue.