Open AccessModification of ribosomal proteins in sea urchin eggs following fertilization
Author(s) -
TAKESHIMA Kazuhito,
NAKANO Eizo
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07847.x
Subject(s) - hemicentrotus , sea urchin , ribosomal protein , human fertilization , embryo , biology , ribosome , ribosomal rna , gel electrophoresis , polyacrylamide gel electrophoresis , microbiology and biotechnology , biochemistry , genetics , rna , enzyme , gene
Analysis of the ribosomal proteins in sea urchin eggs by two‐dimensional polyacrylamide gel electrophoresis revealed postfertilization changes in the proteins of both the small and the large subunits. Five egg‐ribosomal proteins (S7, S16, S19, L19, L31) appeared to undergo rapid changes to the corresponding embryo‐specific proteins. These changes were completed within 30 min after fertilization, and identical electrophoretic patterns were observed among the different developmental stages of embryos. One of the five proteins, S7, showed an increase in the phosphorylated form. The remainder showed qualitative shifts to the corresponding embryo‐specific proteins; however, peptide map analyses revealed the existence of common structural units between the corresponding proteins. These modifications were observed in the three species of sea urchin studied (Pseudocentrotus depressus, Hemicentrotus pulcherrimus and Anthocidaris crassispina) , except in the case of one protein (L31). Purification of ribosomes by different procedures based on high‐salt treatment gave the same results with respect to the egg‐specific and embryo‐specific proteins.