Open AccessIdentification of the α and β subunits of the chloroplast coupling factor one in Chlamydomonas reinhardi
Author(s) -
MERCHANT Sabeeha,
SELMAN Bruce R.
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07838.x
Subject(s) - chlamydomonas , protein subunit , chloroplast , spinach , beta (programming language) , g alpha subunit , alpha (finance) , biology , chemistry , biochemistry , medicine , gene , construct validity , nursing , mutant , computer science , patient satisfaction , programming language
The α and β subunits of the Chlamydomonas reinhardi coupling factor one have been identified by an immunochemical method and by the reaction of dicyclohexylcarbodiimide with the β subunit. Antibodies raised against the C. reinhardi subunit with the highest apparent molecular weight react with the α subunit of spinach coupling factor one and antibodies raised against the β subunit of the spinach chloroplast enzyme cross‐react with the C. reinhardi subunit of lower apparent molecular weight. Dicyclohexylcarbodiimide also reacts with this subunit. We conclude therefore that the two subunits of highest apparent molecular weight can be named α and β in order of decreasing apparent molecular weight, in contrast to the nomenclature suggested by Piccioni, R. G., Bennoun, P. and Chua, N.‐H. [(1981) Eur. J. Biochem. 117 , 93–102].