Open AccessUse of inter‐proton nuclear Overhauser effects to assign the nuclear magnetic resonance spectra of oligodeoxynucleotide and hybrid duplexes in aqueous solution
Author(s) -
REID David G.,
SALISBURY Stephen A.,
BROWN Tom,
WILLIAMS Dudley H.,
VASSEUR JeanJacques,
RAYNER Bernhard,
IMBACH JeanLouis
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07654.x
Subject(s) - nuclear overhauser effect , chemistry , nuclear magnetic resonance , duplex (building) , proton , aqueous solution , spectral line , two dimensional nuclear magnetic resonance spectroscopy , nuclear magnetic resonance spectroscopy , proton nmr , crystallography , stereochemistry , dna , physics , nuclear physics , biochemistry , astronomy
Inter‐proton nuclear Overhauser enhancements (NOEs) have been used to assign the aromatic, anomeric and 2′ resonances in the 1 H nuclear magnetic resonance spectrum of the duplex formed between d(T‐C‐A‐C‐A‐T) and d(A‐T‐G‐T‐G‐A). The same techniques have been applied to assignments in the hybrid duplex formed by d(T‐C‐A‐C‐A‐T) with r(A‐U‐G‐U‐G‐A). Comparison of intra‐residue with inter‐residue NOEs yields structural information which suggests that the conformations of both duplexes are similar. The NOEs are consistent with inter‐proton distances measured from models of B‐form DNA. Circular dichroic results confirm these deductions.