Open AccessDevelopmental changes in poly(A) polymerase activity in Artemia
Author(s) -
SASTRE Leandro,
SEBASTIÀN Jesús
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07618.x
Subject(s) - polymerase , embryo , enzyme , enzyme assay , biology , microbiology and biotechnology , biochemistry
The levels of poly(A) polymerase activity have been determined during Artemia early development. Poly(A) polymerase activity increases steadily during postgastrular embryonic development reaching a maximum shortly after hatching. The rise of poly(A) polymerase is concomitant with an increase in poly(A) content and with a change in the subcellular distribution of the enzyme activity, the major increase corresponding to the nuclear fraction. Only one isoenzyme of poly(A) polymerase has been identified in Artemia embryos and nauplii despite changes in enzyme levels and subcellular changes during early development. Poly(A) polymerase is not associated with the cytoplasmic poly(A)‐containing ribonucleoprotein particles stored in Artemia dormant embryos.