Open AccessA 113 Cd and 1 H NMR Study of the Interaction of Calmodulin with D600, Trifluoperazine and Some Other Hydrophobic Drugs
Author(s) -
ANDERSSON Anita,
DRAKENBERG Torbjörn,
THULIN Eva,
FORSÉN Sture
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07589.x
Subject(s) - calmodulin , trifluoperazine , chemistry , verapamil , binding site , calcium , stereochemistry , biochemistry , organic chemistry
The interaction of calmodulin with D600 (a methoxy derivative of verapamil), trifluoperazine and some other drugs was studied by 113 Cd and 1 H NMR. All four cation binding sites of calmodulin were found to be affected by the binding of the drugs to calmodulin. The physiologically active and inactive forms of felodipine were found to give qualitatively the same changes in the 113 Cd NMR spectra of calmodulin. The interpretation of this observation in terms of the physiological relevance of the binding to calmodulin is discussed. The binding constants for the two strongly bound trifluoperazine molecules were found to differ by one or two orders of magnitude.