Open AccessSomatic Antigens of Pseudomonas aeruginosa
Author(s) -
KNIREL Yuriy A.,
VINOGRADOV Evgeny V.,
SHASHKOV Alexander A.,
DMITRIEV Boris A.,
KOCHETKOV Nikolay K.,
STANISLAVSKY Evgeny S.,
MASHILOVA Galina M.
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07564.x
Subject(s) - polysaccharide , chemistry , monosaccharide , anomer , solvolysis , nuclear magnetic resonance spectroscopy , stereochemistry , carbohydrate conformation , residue (chemistry) , trisaccharide , galactose , biochemistry , hydrolysis
On mild acid degradation of Pseudomonas aeruginosa O:3(a),c, O:3a,d,e and O:3(a),d,f lipopolysaccharides O‐specific polysaccharides were isolated. The trisaccharide repeating units of O:3(a),c and O:3a,d,e polysaccharides contained 2‐acetamido‐2,6‐dideoxy‐ d ‐galactose and 2,3‐(1‐acetyl‐2‐methyl‐2‐imidazolino‐5,4)‐2,3‐dideoxy‐ d ‐mannuronic acid, which were identified previously as the constituents of P. aeruginosa O:3a,b and O:3a,d O‐specific polysaccharides, as well as 2,3‐diacetamido‐2,3‐dideoxy‐ l ‐guluronic acid, which has never before been found in nature. The last monosaccharide was identified without being isolated in the free state, by means of 13 C nuclear magnetic resonance spectroscopy. The structures of O:3(a),c and O:3a,d,e polysaccharides were established by selective hydrogen fluoride solvolysis followed by modification of the trisaccharides obtained and analysis of the 13 C nuclear magnetic resonance spectra at each modification stage. The polysaccharides possessed similar structures of repeating units differing from each other in the anomeric configuration of the N ‐acetylfucosamine residue only:→4)DManImU(β1→4)LGul(NAc) 2 U(α1→3)DFucNAc(β1‐ and →4)DManImU(β1→4)LGul(NAc) 2 U(α1→3)DFucNAc(α1‐,where DManImU = 2,3‐(1‐acetyl‐2‐methyl‐2‐imidazolino‐5,4)‐2,3‐dideoxy‐ d ‐mannuronic acid, LGul(NAc) 2 U = 2,3‐diacetamido‐2,3‐dideoxy‐ l ‐guluronic acid, DFucNAc = 2‐acetamido‐2,6‐dideoxy‐ d ‐galactose. The same components were detected in the O:3(a),d,f polysaccharide but not in the same stoichiometric ratio; this polysaccharide possessed no regular structure. The immunochemical study of lipopolysaccharides of all five P. aeruginosa O:3 serotypes showed a definite interrelation between their serological properties and structures of the corresponding O‐specific polysaccharides.