Open AccessAberrant Migration of Lipopolysaccharide in Sodium Dodecyl Sulfate/Poyacrylamide Gel Electrophoresis
Author(s) -
HITCHCOCK Penny J.
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07517.x
Subject(s) - polyacrylamide gel electrophoresis , gel electrophoresis , sodium dodecyl sulfate , gel electrophoresis of proteins , electrophoresis , two dimensional gel electrophoresis , chemistry , lipopolysaccharide , pulsed field gel electrophoresis , molecular weight size marker , chromatography , polyacrylamide , sodium , gel electrophoresis of nucleic acids , difference gel electrophoresis , microbiology and biotechnology , biochemistry , biology , enzyme , gene , immunology , proteomics , polymer chemistry , organic chemistry , genotype
Purified lipopolysaccharides of salmoellae strains were separated by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Pre‐electrophorees of polyacrylamide gels had no apparent effect on one‐dimensional silver‐stained lipopolysaccharide profiles. However, without pre‐electrophoresis, two‐dimensional and three‐dimensional patterns contained numerous bands with varied migration patterns compared to those in the one‐dimension gels. The lipopolysaccharide was altered within the polyacrylamide gel during electrophoresis. Pre‐electrophoresis of gels eliminated aberrant migration patterns.