Open AccessThe Precursor of Rat Liver Mitochondrial Glutamate Dehydrogenase has Enzymatic Activity
Author(s) -
FELIPO Vicente,
MIRALLES Vicente,
KNECHT Erwin,
HERNANDEZYAGO José,
GRISOLIA Santiago
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07511.x
Subject(s) - glutamate dehydrogenase , reticulocyte , biochemistry , enzyme , lysis , cytosol , glutamate receptor , chemistry , mitochondrion , dehydrogenase , rna , microbiology and biotechnology , biology , receptor , gene
The cytosolic precursor for the mitochondrial glutamate dehydrogenase of rat liver was synthesized in a cell‐free reticulocyte lysate using messenger RNA from rat liver. To check whether this precursor had enzymatic activity, a highly sensitive fluorimetric method, which can measure picogram quantities of enzyme, was used together with competitive dissociation of the precursor from an immunoprecipitate with inactive glutamate dehydrogenase. Glutamate dehydrogenase activity, corresponding to that estimated from incorporation of [ 35 S]‐methionine, was detected in the precursor. The significance of this finding is discussed.