Open AccessThe Stereochemical Course of Nucleotidyl Transfer Catalysed by NAD Pyrophosphorylase
Author(s) -
LOWE Gordon,
TANSLEY Gaynor
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07334.x
Subject(s) - nad+ kinase , nicotinamide , pyrophosphate , stereochemistry , nicotinamide adenine dinucleotide , chemistry , phosphodiesterase , enzyme , nucleotide , biochemistry , nicotinamide mononucleotide , gene
NAD pyrophosphorylase catalyes nucleotidyl trasfer from adensone ( R ′‐[α‐ 17 O]triphosphate to nicotinamide monocucledotide with inversion of configuration at the α‐P giving ( S ‐[ 17 O]NAD + . The simplest interpretation of the observation is that the adenyly group is trasferred directly from ATP to the co‐substrate by an ‘in line’ mechanism. It is also shown that snake venom phosphodiesterase hydrolyses NAD + regio‐specifically at the adenylyl terminus of the pyrophosphate bond.