Open AccessCa 2+ Flux as an Initial Event in Phagocytosis by Rat Kupffer Cells
Author(s) -
BIRMELIN Manfred,
DECKER Karl
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07295.x
Subject(s) - phagocytosis , flux (metallurgy) , event (particle physics) , microbiology and biotechnology , chemistry , biology , physics , organic chemistry , quantum mechanics
Ca 2+ uptake by monolayer cultures of rat Kupffer cells was strongly stimulated immediately after contact with phagocytosable material, e.g. zymosan particles. The intensity of the luminol‐mediated chemiluminescence following zymosan addition was dependent on extracellular Ca 2+ ; addition of the Ca 2+ ‐ionophore A 23187 did not further increase the zymosan‐elicited response. The superoxide‐mediated chemiluminescence was not inhibited by indomethacin but could be suppressed by compounds known to inhibit Ca 2+ ‐calmodulin‐dependent reactions. Analysis of their efficiency, however, suggests that the O − 2 production is mediated by Ca 2+ rather than Ca 2+ ‐calmodulin and that the inhibitors exert another, e.g. membrane‐directed influence.