Open AccessDifferential Response of Cultured Parsley Cells to Elicitors from Two Non‐pathogenic Strains of Fungi
Author(s) -
TIETJEN Klaus G.,
HUNKLER Dieter,
MATERN Ulrich
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07277.x
Subject(s) - elicitor , bergapten , phytophthora megasperma , furanocoumarin , phytoalexin , brefeldin a , phytotoxin , methoxsalen , biology , microbiology and biotechnology , psoralen , biochemistry , chemistry , toxin , cell , golgi apparatus , gene , immunology , resveratrol , dna , psoriasis
Dark‐grown cell suspension cultures of parsley, Petroselinum hortense , produce furanocoumarins after treatment with elicitor preparations of either Phytophthora megasperma f.sp. glycinea (Pmg elicitor) or Alternaria carthami Chowdhury (Ac elicitor). The linear furanocoumarins, psoralen and xanthotoxin, and the benzodipyrandione, graveolone, are the major products synthesized in response to Pmg elicitor, besides small amounts of the furanocomarin bergapten. Treatment with Ac elicitor induces predominantly the formation of bergapten and the furanocoumarin isopimpinellin, as well as small amounts of graveolone. While Pmg elicitor leads to cell death within a few days, cell mass increased for at least 6 days after treatment with Ac elicitor. Brefeldin A, a phytotoxin produced by A. carthami , inhibits growth of parsley cell suspension cultures considerably at a concentration of 0.01 mM and growth of the cells ceased at a concentration of 0.1 mM toxin. Concomitantly, furanocoumarin biosynthesis was suppressed in our system by a concentration of brefeldin A within 0.01–0.1 mM.