Open AccessA Poly(U)‐Binding Factor Stimulating EF‐1 Activity in the Wheat‐Germ Soluble Fraction
Author(s) -
CALDIROLI Ernesto,
ZOCCHI Graziano,
COCUCCI Sergio
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07257.x
Subject(s) - sephadex , fraction (chemistry) , elongation , eukaryotic translation elongation factor 1 alpha 1 , chemistry , column chromatography , affinity chromatography , sepharose , wheat germ , specific activity , chromatography , biochemistry , materials science , enzyme , rna , ribosome , gene , metallurgy , ultimate tensile strength
A poly(U)‐binding activity is present in the high‐speed supernatant fraction of embryo homogenates from wheat seeds. The factor responsible for such activity was found to have a stimulatory effect on the elongation factor 1 (EF‐1). It copurifies with EF‐1 L , the lighter form of EF‐1, through Sephadex G‐200, DEAE‐cellulose, hydroxyapatite and poly(U)‐Sepharose 4B column chromatography. The two factors could be separated only through a heating step which destroyed EF‐1 activity whilst leaving most of the poly(U)‐binding activity unaltered.