Open AccessInhibitory Effect of 1‐β‐ d ‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP
Author(s) -
IUJVIDIN Sonia,
FELEDI Catalina,
MEDRANO Estela E.,
MORDOH José
Publication year - 1983
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1983.tb07150.x
Subject(s) - phosphatidic acid , enzyme , dna synthesis , substrate (aquarium) , biochemistry , chemistry , dna , cytosine , microbiology and biotechnology , biology , phospholipid , ecology , membrane
The synthesis of phosphatidyl‐dCMP in mouse thymocytes is inhibited by the antineoplastic agent 1‐β‐ d ‐arabinofuranosyl cytosine (AraCyt)50% inhibition (ID 50 ) being reached at an AraCyt concentration of 0.18 mM. IN the same cells, ID 50 for DNA synthesis is 0.03 mM. This inhibition is probably mediated by the phosphorylated derivative of AraCyt (aCTP) since the synthesis of phosphatidyl‐dCMP from dCTP using permeabilized thymocytes is inhibited by a aCTP (ID 50 = 0.11 mM). The incorporatioin of [ 3 H]AraCyt inbto the organic phase could also be detected, suggesting that this drug may act as a substrate for the enzyme that catalyzes the transfer of dCTP into phosphatidic acid.