Open AccessLocal Anaesthetics Including Benzyl Alcohol Activate the Adenylate Cyclase in Trypanosoma brucei by a Calcium‐Dependent Mechanism
Author(s) -
VOORHEIS H. Paul,
MARTIN B. Richard
Publication year - 1982
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1982.tb19778.x
Subject(s) - cyclase , adenylate kinase , trypanosoma brucei , chemistry , benzyl alcohol , alcohol , calcium , local anaesthetic , kinetics , receptor , biophysics , biochemistry , stereochemistry , catalysis , biology , organic chemistry , medicine , anesthesia , physics , quantum mechanics , gene
1 The adenylate cyclase of Trypanosoma brucei is activated by local anaesthetics. 2 Activation of adenylate cyclase by the local anaesthetic, benzyl alcohol, requires Ca 2+ . 3 The kinetics of the presteady state reveal that the activation step occurs prior to and is separate from the catalytic step. 4 The activation step in the presence of Ca 2+ can be stimulated some 200‐fold and is highly co‐operative with respect to benzyl alcohol concentration. 5 The mechanism by which local anaesthetics activate may be by exposing the Ca 2+ ‐binding site of the catalytic step. Ca 2+ receptor which is oriented towards the hydrophobic regions of the plasma membrane in the basal state.