Open AccessPancreatic Colipase : Crystallographic and Biochemical Aspects
Author(s) -
PIERROT Marcel,
ASTIER JeanPierre,
ASTIER Martine,
CHARLES Maurice,
DRENTH Jan
Publication year - 1982
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1982.tb19774.x
Subject(s) - colipase , crystallization , micelle , low resolution , residue (chemistry) , chemistry , methionine , crystallography , high resolution , resolution (logic) , biochemistry , pancreatic lipase , amino acid , organic chemistry , computer science , enzyme , remote sensing , artificial intelligence , aqueous solution , lipase , geology
A detailed study of the crystallization of hog and horse colipases has been undertaken. Several crystallographic varieties have been obtained and a 0.3‐nm resolution structure determination is actually in progress. The sequence of the A form of horse colipase (one methionine) is given. From spectrophotometric experiments and sequence comparisons, the involvement of the aromatic residue in position 52 in the miceile binding site has been demonstrated.